期刊论文

中文

食品安全质量检测学报

2095-0381

2017

8

5

1857-1861

10.19812/j.cnki.jfsq11-5956/ts.2017.05.063

本校为第一机构

目的 建立高效液相色谱法检测槟榔中阿斯巴甜的含量的分析方法.方法 槟榔去除卤水及内层纤维后,以磷酸-水溶液(pH=2)为提取液、采用超声法提取槟榔中阿斯巴甜.提取液经Agilent ZORBAX SB-C18(4.6 mm×250 mm,5μm)色谱柱分离,以乙腈-10 mmol/L KH2PO4水溶液(pH=3.5)(14∶86,V∶V)为流动相,流速为1 mL/min,采用紫外检测器在210 nm检测波长下对阿斯巴甜含量进行测定.结果 阿斯巴甜在4.444～222.2 μg/mL范围内呈现良好的线性关系,检出限为0.22 μg/mL,精密度为0.17％,在3个加标水平下的平均回收率为99.80％.结论 本提取方法易于操作、提取率高且稳定性良好,可用于槟榔中阿斯巴甜的提取;本检测方法...

Objective To establish a method for determination of aspartame in areca by high performance liquid chromatography (HPLC).Methods Aspartame was extracted from areca by ultrasound extraction method with phosphoric acid aqueous solutions (pH=2) after removed of brine and inner fiber.The extraction solution was separated by Agilent ZORBAX SB-C18 column (4.6 mm×250 mm,5 μm),using acetonitrile and 10 mmol/L KH2PO4 solution (pH=3.5)(14∶86,V∶ V)as mobile phase with a flow rate of 1 mL/min,and detected by ultraviolet detector at the wavelength of 21...