期刊论文

中文

食品安全质量检测学报

2095-0381

2019

10

18

6195-6200

10.19812/j.cnki.jfsq11-5956/ts.2019.18.040

31671931:国家自然科学基金 31601551:国家自然科学基金

本校为第一机构

目的 比较苎麻籽总黄酮含量的测定方法.方法 以芦丁和槲皮素为对照品, 比较直接测定法、NaNO2-Al3+-NaOH法、AlCl3法3种比色方法下苎麻籽(70%乙醇提取液)的紫外-可见光谱图, 并通过精密度、稳定性、干扰试验、加标回收率试验对所选方法进行评价.结果 芦丁和槲皮素均不适合作为直接测定苎麻籽总黄酮的对照品; NaNO2-Al3+-NaOH法最大吸收波长为500 nm, 但受某些非黄酮类物质干扰, 使得该方法下的总黄酮含量测定值虚高; AlCl3法以芦丁为对照品, 测定波长为401 nm, 在0.004～0.02 mg/mL范围内浓度与吸光度值成良好的线性关系, 相关系数 r2=0.9998, 该法受干扰小, 精密度高, 平均回收率为(99.46±4.1...

Objective To compare the determination methods of total flavonoids in ramie seeds.Methods The ultraviolet-visible spectra of 2 standard reference substance(rutin and quercetin)and total flavonoids(solvent:70%ethanol)in ramie seeds based on the direct colorimetry,NaNO2-Al^3+-NaOH method and AlCl3 method were compared,and the selected method was evaluated by precision,stability,interference test,and recovery test.Results Both rutin and quercetin were not suitable as a standard reference substance for the direct determination of total flavonoids i...