期刊论文

Optimization and Comparison of Alkali Extraction and Acid Extraction of Bound Polyphenols in Psidium guajava Linn

中文

天然产物研究与开发

1001-6880

2018

30

7

1242-1251

10.16333/j.1001-6880.2018.7.025

国家自然科学基金面上项目（31671858） 湖南省教育厅青年基金（14B088）

本校为第一机构

以红心番石榴果肉的冻干粉末为实验材料,先提取游离多酚,剩下的残渣分别采用酸水解和碱水解法提取结合多酚。两种方法在单因素实验基础上,选择浓度、温度、时间这3个影响因素进行响应面设计。采用Design Expert 8.0.6.1软件进行响应面试验设计及数据分析,结果表明温度对结合多酚的得率影响显著,而且酸水解比碱水解获得的结合多酚多。最终确定为:碱水解最佳提取条件:NaOH浓度为7.24 mol/L,温度98.22 ℃,时间24.88 h,此条件下结合多酚得率为1.238%;酸水解最佳提取条件:HCl浓度为4.05 mol/L,温度94.87 ℃,时间29.95 h,此条件下结合多酚得率为2.002%。

In this study,the alkaline hydrolysis and acid hydrolysis conditions for extracting bound polyphenols were optimized with response surface method,after free polyphenols removed from the freeze-dried powder of Psidium guajava.Based on single factor experimental data,influences factors such as solution concentration,hydrolysis temperature and hydrolysis time on the extraction yield of bound polyphenols was optimized with response surface analysis. The optimized alkaline hydrolysis conditions for bound polyphenols were Na OH of 7. 24 mol/L,hydroly...