期刊论文

Study on secondary metabolites of endophytic fungi Strain TPL04 from Tephrosia purpurea

中文

天然产物研究与开发

1001-6880

2019

31

4

633-637

10.16333/j.1001-6880.2019.4.012

湖南省自然科学基金(2017JJ3114) 湖南农业大学自然科学基金(16QN31).

本校为第一机构

从灰毛豆叶片中分离出内生真菌-淡紫拟青霉菌Purureocillium lilacinum (TPL04),对其发酵产物采用硅胶柱色谱、SephadexLH-20凝胶色谱、制备薄层色谱、中压柱色谱及制备液相色谱等方法进行分离纯化,共获得8个单体化合物.通过波谱学方法并结合相关文献数据比对,化合物结构鉴定为5-羟基己酸-4-内酯(1)、(R)-4-苄基-2-噁唑烷酮(2)、2'-脱氧胸苷(3)、对羟基苯甲酸甲酯(4)、光色素(5)、β-胡萝卜苷(6)、尿嘧啶核苷(7)、己六醇(8).其中化合物1、2、4是首次从淡紫拟青霉菌中分离得到,抑菌实验表明化合物4对水稻纹枯菌(Pellicularia sasakii)、辣椒疫霉菌(Phytophthora capsici)、草莓灰霉菌(Botrytis c...

Purureocillium lilacinum (TPL04) is an endophytic fungus isolated from the leaves of Tephrosia purpurea .To investigate the secondary metabolites of the strain (TPL04),the chemical constituents of the fermentation products were isolated by repeated silica-gel column chromatography,Sephadex LH-20,preparative thin layer chromatography,as well as medium pressure column chromatography and preparative HPLC.Finally,eight compounds were obtained,and their structures were elucidated as 5-hydroxyhexan-4-olide ( 1 ),( R )-4-benzyl-2-oxazolidinone ( 2 ),2...