期刊论文

Optimization of fermentation process for L-malic acid with xylose by Aspergiilus parasiticus CICC403653.0, 0.20, 0.15, 0.08 and 80.00 g/L, respectively. The yield of malic acid from the optimal condition was 53.58 g/L and it was 40.5% higher than that of original condition. The reasonable fermentation

中文

微生物学通报

Microbiology

0253-2654

2014

27

6

1052-1062

10.13344/j.microbiol.china.130439

国家自然科学基金项目(No.31071636,31101352)； 安徽省科技计划项目(No.1206c0805017)；

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[目的]为提高L-苹果酸产量及木糖利用率,以寄生曲霉(Aspergillus parasiticusCICC40365)为菌种,木糖为碳源,对其发酵工艺及木糖代谢途径进行初步研究.[方法]采用单因素试验和响应曲面法(Box-Behnken设计)对培养基和发酵条件进行优化.[结果]获得最佳培养基配方为:木糖100.0 g/L、硫酸铵2.0 g/L、酵母浸粉3.0 g/L、硫酸镁0.20 g/L、硫酸锰0.15 g/L、硫酸亚铁0.08 g/L、碳酸钙80.00 g/L,L-苹果酸的产量为53.58 g/L,较优化前提高40.5％.发酵条件较好组合为:接种量为8％(体积比)、摇瓶装液量60 mL/250 mL、发酵温度32℃ 、摇床转速170 r/rmin、发酵周期8d,L-苹果酸的产量为55.47 g/L. Mg2+、Mn2+对木...

[Objective] To increase the yield of L-malic acid and the utilization rate of xylose, the Aspergillus parasiticus CICC40365 was used as the strain to produce L-malic acid with xylose, and the fermentation technology in shake flask was investigated in the work. [Methods] The medium components and fermentation conditions were optimized through single factor experiments and response surface methodology. [Results] The optimal medium components were as follows： the xylose, （NH4）2SO4, yeast extract powder, MgSO4, MnSO4·H2O, FeSO4·7H2O and CaCO3 ...