期刊论文

中文

微生物学通报

0253-2654

2017

44

10

2353-2360

10.13344/j.microbiol.china.160923

国家重点研发计划项目（No.2016YFD0300700） 公益性行业（农业）科研专项项目（No.201203014） 湖南省“十二五”重点学科项目（No.0904）

本校为第一机构

【目的】鉴定湖南省桃江病圃稻瘟病菌无毒基因型,为合理搭配种植湖南省水稻抗瘟品种和抗病育种提供依据。【方法】在湖南桃江病圃采集水稻品种"丽江新团黑谷"(LTH)稻瘟菌病样,用单孢分离法分离稻瘟病菌单孢并纯化获得单孢菌株,用针刺离体法将菌株接种到以"LTH"为轮回亲本培育而成的24个含单抗瘟基因的水稻5叶期第5叶片上,对供试菌株进行无毒基因鉴定,并应用联合致病性系数和联合抗病性系数分析抗瘟基因组合间的互作。【结果】供试92个稻瘟病单孢菌株含有全部的24个无毒基因,对24个已知含单抗瘟基因的水稻材料表现出不同程度的毒力水平,含水稻抗瘟基因Pi-20对供试菌株抗菌频率最高,达54.35%;通过...

[Objective] We identified avirulence genes of rice blast fungus in Taojiang, Hunan Province, to deploy rice blast resistant varieties and blast resistant breeding in Hunan. [Methods] We collected samples of leaf blast of Lijiangxintuanheigu (LTH) in Taojiang and isolated blast fungi through single spore isolation. Avirulence genes of these fungi were identified by in vitro after being inoculated onto the 5th leaf of the 24 monogenic near-isogenic lines against blast at 5 leaves stage which is cultivated from LTH with known blast-resistance gene...