期刊论文

中文

微生物学通报

0253-2654

2022

49

2

505-513

10.13344/j.microbiol.china.210506

国家自然科学基金（31672539）～～；

本校为第一机构

【背景】碱性磷酸酶作为工具酶被广泛应用于各个领域,在免疫学检测方面应用较多的是PhoA家族的碱性磷酸酶,尚无关于PhoD家族的碱性磷酸酶在免疫学检测方面的研究。【目的】筛选出一株产高酶活性PhoD家族碱性磷酸酶的细菌,并将其phoD基因进行克隆表达,研究PhoD的酶学性质,为PhoD家族的碱性磷酸酶在免疫学检测方面的应用奠定一定的基础。【方法】采取有机质丰富的土样在有机磷平板中进行细菌分离,以4-硝基苯磷酸二钠盐(4-nitrophenyl phosphate disodium salt hexahydrate,p-NPP)为底物测定有机磷平板中单菌落的酶活性,选取酶活性高的菌株作为目的菌株,克隆其phoD基因。【结果】筛选到一株产碱性磷...

[Background] Alkaline phosphatase(ALP),a tool enzyme,is widely used in various fields. Among the ALPs,PhoA has been extensively applied in immunological detection,while the functions of PhoD in immunological detection have been rarely reported.[Objective] This paper aims to screen a bacterial strain producing high-activity PhoD,clone and express the enzyme gene phoD,and study the enzymatic properties of PhoD,which is expected to lay a foundation for the application of PhoD in immunological detection.[Methods] Soil samples rich in organic matter...