National Natural Science Foundation for Young Scientists of ChinaNational Natural Science Foundation of China (NSFC) [30901090]; Planned Science and Technology Project of Hunan Province, China [2014FJ2011]; Scientific Research Fund of Hunan Provincial Education DepartmentHunan Provincial Education Department [09B046]
机构署名：
本校为第一且通讯机构
院系归属：
动物医学院
摘要：
The use of serologically detectable male (SDM; also called H-Y) antigens to identify male embryos may be limited by the source of anti-SDM antibody. In the present study, novel anti-SDM B9-Fab recombinant clones (obtained by chain shuffling of an A8 original clone) were used to detect SDM antigens on murine embryos. Murine morulae and blastocysts (n=138) were flushed from the oviducts of Kunming mice and incubated with anti-SDM B9-Fab for 30 min at 37°C. With an indirect immunofluorescence assay, the membrane and inner cell mass had bright gre...
