National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30671548]
机构署名:
本校为第一且通讯机构
院系归属:
动物医学院
摘要:
Penicillic acid is one of the main mycotoxins in moldy feedstuff and has toxic effect on livestock and poultry and probably humans due to food chain transmission. The objective of this study was to generate and characterize a monoclonal antibody to penicillic acid for the efficient detection of penicillic acid from Penicillium cyclopiumby immunological methods. To this end, penicillic acid was conjugated to bovine serum album (BSA) using the Mannich reaction and coupled with ovalbumin (OVA) by the method of 1-ethyl-3-(3-dimethyl-aminopropyl) ca...