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A near-infrared fluorescent probe for imaging cysteine changes in Arabidopsis thaliana

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成果类型:
期刊论文
作者:
Yuan, Yao;Cao, Fuxiang
通讯作者:
Cao, FX
作者机构:
[Cao, FX; Yuan, Yao; Cao, Fuxiang] Cent South Univ Forestry & Technol, Coll Chem & Chem Engn, Changsha 410004, Hunan, Peoples R China.
[Cao, FX; Cao, Fuxiang] Hunan Agr Univ, Coll Hort, Changsha 410128, Hunan, Peoples R China.
通讯机构:
[Cao, FX ] C
Cent South Univ Forestry & Technol, Coll Chem & Chem Engn, Changsha 410004, Hunan, Peoples R China.
Hunan Agr Univ, Coll Hort, Changsha 410128, Hunan, Peoples R China.
语种:
英文
期刊:
NEW JOURNAL OF CHEMISTRY
ISSN:
1144-0546
年:
2025
卷:
49
期:
9
页码:
3599-3605
机构署名:
本校为通讯机构
院系归属:
园艺园林学院
摘要:
In this work, a robust "turn-on" NIR fluorescent probe Cx-Cys was constructed for cysteine detection and imaging in Arabidopsis thaliana. Specifically, a 1,8-naphthalimide-based NIR fluorophore was used as the signaling group, while the acrylate group served as the recognition moiety for cysteine as well as the fluorescence quenching group. The probe shows a remarkable NIR response toward cysteine in the infrared region (718 nm), and a large Stokes shift (103 nm). Cx-Cys displayed high sensitivity and selectivity to cysteine with low detection limits of 73 nM over other amino acids and bio-thi...

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