期刊论文

Improvement of Blast Resistance and Low Cadmium Accumulation of Dual-purpose Genic Male Sterile Rice Line Chuang 5S by Molecular Marker-assisted Selection

中文

杂交水稻

1005-3956

2019

34

4

51-56

10.16267/j.cnki.1005-3956.20190326.078

国家重点研发计划项目(2016YFD0101100) 湖南省科技重大专项(2015NK1001-1).

本校为第一机构

为了培育抗稻瘟病且籽粒镉低积累的水稻两用核不育系,利用谷梅4号、天津野生稻和魔王谷为稻瘟病抗源, Cd14030(日本晴的衍生系)为稻米镉低积累基因OsHMA3的供体亲本,采用分子标记辅助选择技术和逐步聚合的方法,创制了基于创5S(C5S)背景的包含抗病双基因和镉低积累基因的三基因聚合系。三基因聚合系的苗瘟感病级别为1.5~2.1级,与抗病双基因聚合系无显著差异,但三基因聚合改良株系的糙米镉含量因亲本来源而异,其中,基因型为C5S(Pi49+Pigm+OsHMA3)的改良系糙米镉含量为0.051~0.067 mg/kg,与日本晴(0.051 mg/kg)相当,较C5S(0.118 mg/kg)下降43.2%~56.8%,较好地将稻瘟病抗性与镉低积累特性聚合于两用...

To develop the dual-purpose genic male sterile rice lines with both low grain cadmium accumulation and blast resistance, the improved three-gene polymerized lines of Chuang 5S (C5S), containing two blast resistance genes and a low cadmium accumulation gene, were successfully developed with Gumei 4 (Pigm), Tianjinyeshengdao (Pi2-1) and Mowanggu (Pi49) as blast resistance sources and Cd14030 (a derivative of Nipponbare) as the donor parent of the low cadmium accumulation gene OsHMA3, through the molecular marker-assisted selection (MAS) technology and the progressive aggregation method. The fiel...