期刊论文

Effects of Rice Glycoside Hydrolase Gene OsINV3 on Pollen Fertility

中文

杂交水稻

1005-3956

2024

39

1

35-43

10.16267/j.cnki.1005-3956.20230219.051

2021NK1002:湖南省科技创新计划岳麓山种业创新项目 kq2004053:长沙市科技计划项目

本校为第一机构

花粉育性是水稻生殖发育中的重要性状,直接影响水稻的产量.本研究鉴定到一个包含Glyco_32核心结构域的糖苷水解酶编码基因OsINV3.通过实时定量PCR对OsINV3的时空表达模式分析发现,该基因在穗发育各阶段均有表达且在幼穗分化第6期具有较高的表达水平.通过亚细胞定位分析,发现OsINV3定位于细胞质.利用CRISPR/Cas9技术获得了粳稻9522和籼稻五山丝苗背景下的功能缺失突变体952201590-1和952201590-2、WSSM01590-1和WSSM01590-2.通过对表型分析及农艺性状统计发现,2种不同背景的突变体材料均表现为株高矮化、花粉育性下降、结实率降低.进一步分析发现,不同背景的突变材料,对育性下降的程度存在差异,在...

Pollen fertility is an important character in rice reproduction and development,which directly affects rice yield.A glycoside hydrolase-encoding gene OsINV3 containing Glyco_32 core domain was identified.Temporal and spatial expression pattern analysis of OsINV3 by real-time quantitative PCR showed that OsINV3 gene was expressed at all stages of panicle development and had a high expression level in the 6th stage of young panicle differentiation.Subcellular localization analysis showed that OsINV3 was localized in cytoplasm.The non-functional mutants 952201590-1 and 952201590-2,WSSM01590-1 and...