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Identification of a Rice Leaf Width Gene Narrow Leaf 22 (NAL22) through Genome-Wide Association Study and Gene Editing Technology

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成果类型:
期刊论文
作者:
Xu, Yuchen;Yan, Shuangyong;Jiang, Su;Bai, Lu;Liu, Yanchen;...
通讯作者:
Houxiang Kang
作者机构:
[Xu, Yuchen; Jiang, Su; Xiao, Yinghui; Peng, Shasha] Hunan Agr Univ, Coll Agron, Changsha 410128, Peoples R China.
[Xu, Yuchen; Peng, Shasha; Liu, Yanchen; Bai, Lu; Chen, Rubin; Liu, Qi; Kang, Houxiang] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China.
[Yan, Shuangyong] Tianjin Acad Agr Sci, Tianjin Crop Res Inst, Tianjin Key Lab Crop Genet Breeding, Tianjin 300112, Peoples R China.
通讯机构:
[Houxiang Kang] S
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China<&wdkj&>Author to whom correspondence should be addressed.
语种:
英文
关键词:
Cell division;CRISPR/Cas9 gene editing;Genetic architecture of rice leaf width;Genome-wide association study;Gibberellin;Rice leaf width;Vein width
期刊:
International Journal of Molecular Sciences
ISSN:
1422-0067
年:
2023
卷:
24
期:
4
页码:
4073-
基金类别:
Conceptualization, H.K. and Y.X. (Yuchen Xu); writing—original draft, Y.X. (Yuchen Xu); writing—review & editing, H.K. and Y.X. (Yuchen Xu); funding acquisition, H.K. and S.Y.; formal analysis, H.K., Y.X. (Yuchen Xu), Y.L., S.P. and Q.L.; investigation, Y.X. (Yuchen Xu), S.Y., S.J., L.B. and R.C.; visualization, Y.X. (Yuchen Xu) and L.B.; validation, Y.X. (Yuchen Xu) and L.B.; project administration, H.K. and Y.X. (Yinghui Xiao). All authors have read and agreed to the published version of the manuscript. This work was financially supported by the International Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (CAASTIP) (CAAS-ZDRW202108), the National Natural Science Foundation of China to Houxiang Kang (32261143468 & 31772120), and the Key Technologies R&D Program of Tianjin to Shuangyong Yan (18YFZCNC01210).
机构署名:
本校为第一机构
院系归属:
农学院
摘要:
Rice leaf width (RLW) is a crucial determinant of photosynthetic area. Despite the discovery of several genes controlling RLW, the underlying genetic architecture remains unclear. In order to better understand RLW, this study conducted a genome-wide association study (GWAS) on 351 accessions from the rice diversity population II (RDP-II). The results revealed 12 loci associated with leaf width (LALW). In LALW4, we identified one gene, Narrow Leaf 22 (NAL22), whose polymorphisms and expression levels were associated with RLW variation. Knocking out this gene in Zhonghua11, using CRISPR/Cas9 gen...

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