Objective:To establish a method that using chlorogenic acid as internal standard to simultaneous determination of chlorogenic acid,5-caffeoylquinic acid,4-dicaffeoylquinic acid,isochlorogenic acid B,isochlorogenic A,isochlorogenic C six contents of major polyphenolic components in Stevia rebaudiana.Methods:To verify the applicability of the method,the methodological investigation of the detection method was carried out.The durability of the relative correction factors was verified by examining different chromatographs and different columns.Fifteen batches of S.rebaudiana raw materials were ana...