期刊论文

中文

激光生物学报

1007-7146

2011

20

6

824-829

10.3969/j.issn.1007-7146.2011.06.022

湖南省科技厅创新项目（2010KJ106） 陕西省教育厅专项基金资助项目（01jk099）

本校为第一机构

以拉曼被孢霉（Mortierella ramanniana）为出发菌株M_5，通过原生质体制备，经亚硝酸和激光等进行复合诱变；进行初筛，经摇瓶发酵法复筛，并测定其相关性能指标，获得一生产性能比出发菌株显著提高的突变株M_(F10)；其干菌体收率为46.8 g/L、油脂产率达34.6 g/L、γ-亚麻酸的产率达12.5 g/L，分别是出发菌株的1.73倍、2.02倍和2.6倍。通过基因传代实验，说明突变株的基因可稳定遗传。

A mutant strain M_（F10） accumulating a large amount of lipid was successfully obtained when the original strain Morlicrella ramanniana-M_5 was treated by compound mutagenesis through the use of HNO_2 and laser after its cell walls was decomposed.The content of lipid in M_（F10） mycelium culture in shaking flask with its all dry cell weight was up to 46.8 g/L which is 173%higher than that in the original strain,and its lipid yield 34.5 g/L andγ-Linolenic acid yield 12.5 g/L are 202%and 260%higher than its original strain.The passing generation lest sh...