期刊论文

中文

激光生物学报

1007-7146

2015

24

5

490-494

10.3969/j.issn.1007-7146.2015.05.016

(2011 GA770007):国家星火计划重大项目 (2010FJ1007-2):湖南省重大科技专项

本校为第一机构

目的：研究茶多酚对氧化脂肪-蛋白质体系的作用机理。方法：利用分光光度计和红外光谱检测法对冻藏条件下氧化脂肪-蛋白质模拟体系中肌原纤维蛋白巯基含量、表面疏水性和二级结构进行研究。结果：在冻藏过程中,添加氧化6 h鱼油的肌原纤维蛋白液,巯基含量的减少和表面疏水性的增加最显著,其减少值和增加值分别为28.62 %和53.72 %;同时β-折叠、α-螺旋的减少和β-转角、无规卷曲的增加也最明显。添加0.05%茶多酚的三个组合与其相应的对照组相比,巯基含量的减少、表面疏水性的增加和二级结构的改变都较为缓慢,其中茶多酚抑制巯基含量减少的效果十分显著。结论：氧化鱼油能加速蛋白质的变性,且氧化...

Objective: To study the effects of tea polyphenols on the oxidation of fat-protein system. Methods: Sulfhydryl content,surface hydrophobicity and secondary structure of the myofibrillar protein of the oxidation fat-protein system in frozen storage conditions had been studied by spectrophotometer,fluorescence spectrophotometer and infrared spectrometer. Results: On frozen-storage conditions,in the myofibrillar protein liquid,which have added oxidated 6 h fish oil,the decrease of sulphur content and increase of the surface hydrophobicity are most...