Transcriptional study of the enhanced epsilon-poly-L-lysine productivity in culture using glucose and glycerol as a mixed carbon source.

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成果类型：

期刊论文

作者：

Zeng, Xin;Miao, Wenyun;Wen, Beibei;Mao, Zhonggui;Zhu, Mingzhi*;...

通讯作者：

Chen, Xusheng;Zhu, Mingzhi

作者机构：

[Zeng, Xin] Huaibei Normal Univ, Coll Life Sci, Huaibei 235000, Peoples R China.

[Chen, Xusheng; Zeng, Xin; Mao, Zhonggui] Jiangnan Univ, Sch Biotechnol, Key Lab Ind Biotechnol, Minist Educ, 1800 Lihu Rd, Wuxi 214122, Jiangsu, Peoples R China.

[Wen, Beibei; Zhu, Mingzhi] Hunan Agr Univ, Key Lab Tea Sci, Minist Educ, Changsha 410128, Hunan, Peoples R China.

[Miao, Wenyun] Rizhao Maternal & Child Care Serv Hosp, Family Planning Serv Ctr, Rizhao 276826, Peoples R China.

通讯机构：

[Chen, Xusheng] J

[Zhu, Mingzhi] H

Jiangnan Univ, Sch Biotechnol, Key Lab Ind Biotechnol, Minist Educ, 1800 Lihu Rd, Wuxi 214122, Jiangsu, Peoples R China.

Hunan Agr Univ, Key Lab Tea Sci, Minist Educ, Changsha 410128, Hunan, Peoples R China.

语种：

英文

关键词：

epsilon-Poly-l-lysine;Fermentation;Mixed carbon source;Transcriptome analysis;Cell physiology

期刊：

Bioprocess and Biosystems Engineering

ISSN：

1615-7591

年：

2019

卷：

期：

页码：

555-566

DOI：

10.1007/s00449-018-2058-1

基金类别：

National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31671846, 31301556]; Science and Technology Department of Jiangsu Province [BY2016022-25]; Open Project Program of the Key Laboratory of Industrial Biotechnology, Ministry of Education, ChinaMinistry of Education, China [KLIBKF201302]; Jiangsu Province Collaborative Innovation Center for Advanced Industrial Fermentation Industry Development Program; Innovation Team of Scientific Research Platform of Anhui Province, China [KJ2015TD001]; China Postdoctoral Science FoundationChina Postdoctoral Science Foundation [2018M632962]

机构署名：

本校为通讯机构

院系归属：

园艺园林学院

摘要：

A glucose–glycerol mixed carbon source (MCS) can substantially reduce batch fermentation time and improve ε-poly-l-lysine (ε-PL) productivity, which was of great significance in industrial microbial fermentation. This study aims to disclose the physiological mechanism by transcriptome analyses. In the MCS, the enhancements of gene transcription mainly emerged in central carbon metabolism, l-lysine synthesis as well as cell respiration, and these results were subsequently proved by quantitative real-time PCR assay. Intracellular l-lysine dete...

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Transcriptional study of the enhanced epsilon-poly-L-lysine productivity in culture using glucose and glycerol as a mixed carbon source.

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