期刊论文

Xiao, L.-T.

中文

植物生理与分子生物学学报

1671-3877

2011

47

1

91-96

10.13592/j.cnki.ppj.2011.01.006

国家自然科学基金项目（30900099）； 湖南省自然科学基金项目（09JJ3052）； 湖南湖南省教育厅青年基金项目（03B011）；

本校为第一且通讯机构

为了研究高钾植物商陆高亲和性K+吸收机制,本试验选用野生商陆为材料,以拟南芥、冰叶日中花、小麦、大麦和水稻等多种植物的HAK家族基因的保守序列,设计简并引物,获得了981 bp的PaHAK1的基因片段.然后应用RACE技术克隆到PaHAK1基因序列,其cDNA全长为2 337 bp,编码771个氨基酸.该编码蛋白质分子量为86.66 kDa,等电点为7.54.蛋白质疏水性和拓扑结构分析显示该氨基酸序列共有12~13个跨膜区.该cDNA序列与冰叶日中花HAK1基因序列相似性高达88%.Real-Time PCR分析表明PaHAK1主要在商陆根中表达,低钾状态可以诱导PaHAK1的高表达

To study the mechanism of high affinity potassium absorption in some high-K plants, the PaHAK1 cDNA fragment of 981 bp sequences was cloned from wild Phytolacca acinosa Roxb. by polymerase chain reaction with degenerate primers designed according to conserved HAKs cDNA sequences of Arabidopsis thaliana, Mesembryanthemum crystallinum, Triticum aestivum, Hordeum vulgare and Oryza sativa. The full-length cDNA sequences of PaHAK1 was 2337 bp which was isolated with rapid amplification of cDNA ends method. The deduced protein was 771 amino acids with molecular weight 86.66 kDa and isoelectric point...