期刊论文

Molecular cloning and expression profile of BnaGPDH gene in Brassica napus

Xiong, X.-H.

中文

植物生理与分子生物学学报

1671-3877

2017

53

9

1772-1780

10.13592/j.cnki.ppj.2017.0189

国家重点研发计划项目 Natural Science Foundation of Hunan Province Science and Technology Program of Hunan Province 湖南省科技计划项目 湖南省自然科学基金 国家重点基础研究发展计划(973计划)项目 This work was supported by the National Key Basic Research Program of China National Key Re-search and Development Program of China

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甘油三磷酸脱氢酶(GPDH)是三酰甘油(TAG)合成的关键酶。为了阐明甘蓝型油菜(Brassica napus) GPDH (BnaGPDH)的表达特性,本研究采用反转录PCR (RT-PCR)方法,获得BnaGPDH基因的3条全长编码序列(CDS),分别命名为BnaGPDH1、 BnaGPDH2、BnaGPDH3,长度分别为1 395、1 395、1 389 bp。生物信息学分析表明, BnaGPDHs具有GPDH基因家族的特征, BnaGPDHs与其他植物GPDHs有高度同源性。时空表达分析表明, BnaGPDHs是组成型表达基因,在花中的表达量最高,叶中的表达量最低。BnaGPDHs在幼嫩种子发育的第5周表达量普遍提高,在果荚发育到第5周表达量明显降低。逆境分析表明, BnaGPDHs响应多种逆境胁迫,可能与植物...

Glycerol-3-phosphate dehydrogenase (GPDH) is a key enzyme in triacylglycerol (TAG) biosynthesis. In order to elucidate the expression feature of BnaGPDHs in Brassica napus, three GPDH full-length coding sequences (1395, 1395 and 1389 bp) were obtained by reverse transcription PCR, and designated as BnaGPDH1, BnaGPDH2 and BnaGPDH3, respectively. Bioinformatic analysis shows that they have the characteristics of the GPDH family, and amino acid sequences of BnaGPDHs are highly homologous to GPDHs in other plants. Spatio-temporal expression results...