期刊论文

Organ characteristics and expression analysis of polycomb subunit KsRING1 in Kalanchoe serrata

Chen, D.-H.

中文

植物生理与分子生物学学报

1671-3877

2018

54

9

1441-1450

10.13592/j.cnki.ppj.2018.0243

浙江农林大学科研发展基金人才启动项目（102/20340-20128） 国家重点研发课题（2017YFC1702201）

本校为第一机构

在拟南芥中,多梳蛋白抑制复合体1 （PRC1）的核心组分AtRING1作为重要的表观遗传因子,其功能缺失导致植物在营养阶段产生胚性愈伤结构,这与落地生根叶生无性苗的发生相类似。本研究首先系统观察了锯缘落地生根主要器官的结构特征,探讨了锯缘落地生根整体发育模式。然后通过同源克隆技术获得2个AtRING1在锯缘落地生根的同源基因并命名为KsRNG1a和KsRING1b,GenBank登录号分别为MH144360和MH144361。实时定量PCR结果表明, KsRING1a/b基因广泛表达于锯缘落地生根的不同组织器官中,表明其功能贯穿于植物生长发育的整个时期。特别是KsRING1a/b的表达量在叶缘无性苗中的表达最高,这与拟南芥同源基因AtR...

In model plant Arabidopsis thaliana, AtRING1 is the core subunit of the Polycomb repressive complex 1 (PRC1) and acts as an important epigenetic factor; its knockout results in the production of embryogenic callus during the vegetative stage, somewhat similar to the emergence of asexual seedlings in K. serrata. In this study, we first systematically analyzed the structural characteristics of the main organs and explored the overall development pattern in K. serrata. Two AtRING1 homologous genes in K. serrata were then obtained by homologous clo...