期刊论文

Cloning and functional characterization of an ASR gene (DiASR1) from dove tree (Davidia involucrata Baill.)

Li, M.

中文

植物生理与分子生物学学报

1671-3877

2019

55

11

1673-1684

10.13592/j.cnki.ppj.2019.0197

湖南省重点研发计划项目（2017NK2252） 湖南省教育厅优秀青年项目（17B282）~~

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ASR蛋白是近年来发现的一种受脱落酸、胁迫或成熟诱导表达的蛋白,研究表明该蛋白在植物的生殖发育和逆境胁迫响应中发挥重要作用。本研究小组前期发现一个编码ASR蛋白的珙桐(Davidia involucrata)基因DiASR1在其苞片中特异表达,且其表达量在苞片发育过程中持续上升,推测其可能在珙桐苞片发育过程中发挥重要作用。本研究克隆得到DiASR1基因,并通过遗传转化在本氏烟草(Nicotiana benthamiana)中超量表达目的基因以分析该基因的功能。对转基因烟草植株进行形态学观察发现,相较于野生型植株,转基因植株生物量积累少,营养生长时间明显缩短,提前进入生殖生长,且转基因植株的花色发生变化。另一方面,转...

ASR (abscisic acid-stress-ripening) protein is a recently identified regulating factor involved in stress resistance and fruit ripening in plants. The expression of ASR protein could be induced by abscisic acid, stress or ripening. Extensive documents indicate that ASR protein plays an important role in reproductive development and response to abiotic stress. In this study, we identified an ASR gene from dove tree (Davidia involucrata) according to the transcriptome data of Davidia bract. The expression pattern of the target gene was bract-spec...