期刊论文

Identification and bioinformatics analysis of CAX family genes and their expression response to Cd~(2+) stress in Brassica napus

Zhang, Z.-H.

中文

植物生理与分子生物学学报

1671-3877

2019

55

5

596-608

10.13592/j.cnki.ppj.2018.0481

国家重点研发计划(2017YFD0200103) 国家自然科学基金(31801923、31101596、31372130) 湖南农业大学‘双一流’建设(kxk201801005).

本校为第一且通讯机构

CAXs (Cation/H~+exchanger antiporter)是一类重要的阳离子跨膜转运蛋白,在调控植物Ca~(2+)平衡,参与转运金属离子Cd~(2+)和Mn~(2+)中发挥了重要作用,但在甘蓝型油菜中尚缺乏系统研究。本文利用生物信息学的方法对甘蓝型油菜CAX家族成员的基因拷贝数、系统进化、进化选择压力、分子特征、保守基序、染色体定位、基因结构及其启动子区域所能结合的顺式作用元件进行了预测和分析;同时采用荧光定量PCR分析了甘蓝型油菜BnaCAXs的组织表达模式及其对Cd~(2+)胁迫的响应。结果表明,甘蓝型油菜CAX家族共包含17个成员,系统进化分析结果表明BnaCAXs与拟南芥进化相似,并且明显地分为5个亚家族。BnaCAXs家族...

CAXs (Cation/H+ exchanger antiporter) are a class of secondary energised ion transporter that primarily functioning in mediating Ca2+ transportation, which play an important role in transporting Cd2+ and Mn2+. However, they are rarely studied in Brassica napus. In this study, the bioinformatical methods were employed to predict and analyze some bioinformatical parameters of BnaCAXs, including copy number, phylogenetic analysis, evolutionary selection pressure, molecular characteristics, conserved motifs, chromosome location, gene structures and...