期刊论文

Effect of plant growth regulator 2,4-D on viscoelasticity of tobacco BY-2 cells

Zhou, T.

中文

植物生理与分子生物学学报

1671-3877

2020

56

5

1053-1063

10.13592/j.cnki.ppj.2019.0272

31870933:国家自然科学基金 12W004:湖南省教育厅委托项目

本校为第一机构

本文基于多次泛音、宽频耗散型石英晶体微天平技术,首次检测了2,4-D作用于模式植物烟草BY-2细胞下细胞壁与细胞骨架粘弹性的动态变化规律。结果表明,0.5 mg·L~(-1) 2,4-D处理下烟草BY-2细胞壁与细胞骨架均变软,在光学显微镜下观察到细胞内物质运输变快。此外,比较了不同浓度(0.1~10 mg·L~(-1))2,4-D对BY-2细胞粘弹性的影响,在1 mg·L~(-1) 2,4-D处理下BY-2细胞的粘弹性变化最明显。

Based on the technique of multiple overtones and wide frequency quartz crystal microbalance with dissipating monitoring (QCM-D), the dynamic changes in viscoelasticity of cell wall and cytoskeleton of model plant tobacco BY-2 cells under the treatments of 2,4-D were monitored for the first time. The results showed that 0.5 mg•L-1 2,4-D softened both the cell wall and cytoskeleton of tobacco BY-2, and the transport of intracellular material became faster as observed under the light microscope. In addition, the effects of different concentration...