期刊论文

Zhou, S.

中文

植物生理与分子生物学学报

1671-3877

2022

58

4

788-796

10.13592/j.cnki.ppj.2021.0382

本校为第一机构

为提高辣椒E29子叶外植体遗传转化效率,本研究建立了从E29子叶外植体愈伤组织中获得植株的高效离体再生体系,并优化了子叶外植体抗性愈伤组织获得的条件。结果表明,在添加2.0 mg·L~(-1) 6-BA和0.5 mg·L~(-1) IAA的MS培养基中,大于98%的子叶外植体切口处可诱导愈伤组织。添加0.1 mg·L~(-1) 2,4-D的MS培养基能高效促进愈伤组织增殖。添加9.0 mg·L~(-1) 6-BA的MS培养基中,愈伤组织诱导芽点效果最好, 20 d后芽点诱导率达11.27%。添加3.0 mg·L~(-1) 6-BA、0.1 mg·L~(-1) IAA及0.3 mg·L~(-1) GA3的MS培养基中,不定芽的伸长效果最佳,平均每块带芽点愈伤组织上可以产生2.01个伸长的不定芽。不添加...

In order to improve genetic transformation efficiency of cotyledon explants of pepper E29, a high-frequency regeneration from cotyledon-induced callus was established and the resistant callus formation protocol was optimized. The result showed that more than 98% response in calli induction from the cuts of cotyledons explants on MS medium containing 2.0 mg•L-1 6-benzylaminopurine (6-BA) and 0.5 mg•L-1 indolyl-3-acetic acid (IAA); The calli were successfully proliferated on MS medium containing 0.1 mg•L-1 2,4-dichlorophenoxyacetic acid (2,4-D...