期刊论文

Zhu, M.

中文

植物生理与分子生物学学报

1671-3877

2022

58

6

1153-1160

10.13592/j.cnki.ppj.100005

上海市绿化和市容管理局科技攻关项目(G212403)。

本校为其他机构

八仙花(Hydrangea macrophylla)花序硕大,花色多样,变种多,观赏价值高,国内外广泛栽培。本研究开展了八仙花的一步成苗离体培养及组织器官激素富集研究。结果表明:MS+1.0 mg·L~(-1) 6-BA+0.2 mg·L~(-1) NAA为八仙花茎段一步成苗诱导最佳配方,首次建立了八仙花一步成苗离体再生技术体系。八仙花受试组织器官6-BA富集水平依次为叶片>茎段>叶柄>茎尖,NAA富集水平依次为叶片>叶柄>茎尖>茎段。本实验在同一培养基条件下,受试材料活力高,无需更换培养基,由外植体一次性发育为完整植株,操作简单,成本低廉,适宜规模化生产。

Hydrangea macrophylla with huge inflorescences and various colors has high ornamental value and is widely cultivated all over the world. This study carried out the one-step seedling culture of hydrangea in vitro and the characteristics of hormone enrichment in different tissue organs. The results showed that MS+1.0 mg•L-1 6-BA+0.2 mg•L-1 NAA was an excellent one-step seedling formation induction formula, with stems of hydrangea as explants. This research established a technical system for one-step regeneration of hydrangea seedlings in vitro ...