期刊论文

中文

江西农业大学学报

1000-2286

2006

28

2

222-225

10.3969/j.issn.1000-2286.2006.02.015

湖南省政府基金（A-15）； 中南林学院***科研启动基金（101-0288）共同资助；

本校为其他机构

应用AFLP技术，筛选了40对引物，其中EcoRI AGC＋MseI CGA，EcoRI AGC＋MseI CAC，EcoRI AGG＋MseI CAC，EcoRI AGG＋MseI CCA，EcoRI ACG＋MseI CAC，EcoRI ACG＋MseI CCA等共12对引物经PCR扩增，效果理想，共扩增出4227条带，带型清晰、丰富、可靠性强。在EcoRl—ACG＋MseI—CAC这对引物下，果实性状优良的单株变异与普通对照（即普通冰糖橙）有明显的带型差异，多态性比例达10．2％。应用AFLP技术进行柑橘芽变的分析和鉴定获得成功。

Forty pairs of AFLP primers were adopted in the AFLP analysis. 4227 clean and stable bands were detected by twelve pairs of AFLP primers which were selected from 40 pairs of primers. There was obvious band difference between common Bingtang Orange and its bud mutations and excellent fruit under the AFLP primers EcoRI - ACG ＋ MseI - CAC , and the polymorphic rate was 10.2%. The application of AFLP in t...