In order to study separation of resin elution test, isolation procedure of flex paraguariensis polyphenols with macroporou resin and antibacterial activities of total and fractional polyphenols were studied. The results showed that HPD500 was optimal absorbent to isolate llex paraguariensis polyphenols. Three fractions with different polarity from IP polyphenols were obtained after gradient elution with aqueous alcohol, llex paraguariensis polyphenols showed considerable antibacterial effects and were more effective against solmonella paratyhpi than tea ployphenols. The antibacterial activity ...