期刊论文

中文

江西农业大学学报

1000-2286

2008

30

4

623-627

10.3969/j.issn.1000-2286.2008.04.010

湖南省重大科技专项（04NK1005）；

本校为第一机构

用携带TERF1基因的EHA105和LB4404 2种农杆菌分别转化糖橙实生苗节间茎段。根据已建立的再生体系和转化体系,对已得到的抗性芽经PCR和RT-PCR检测,验证已获得7个转TERF1基因的糖橙株系。此外,还比较了2种农杆菌菌株对糖橙的浸染能力,结果表明,EHA105农杆菌的浸染能力强于LB4404.

After establishing the regeneration and transformation systems of citrus, the TERF1 gene was mediated into ssweet orange by EHA105 and LB4404 Agrobacterium tumefaciens. Seven plants were verified by PCR and RT- PCR amplification. The results showed that EHA105 had more efficiency in impr...