[Objective]This study aims to investigate the symbiotic relationship between microalgae and Bacillus subtilis and their impact on soil bacterial communities.[Method]Four treatments,including microalgae,Bacillus subtilis,microalgae and Bacillus subtilis,and blank control,were designed.They were continuously cultured at constant temperature in room for 35 days.Soil chemical properties were determined.Using molecular biology techniques such as fluorescence quantitative PCR and Illumina Miseq sequencing,the succession rules of soil bacterial community abundance and composition were analyzed,and th...