期刊论文

Genetic diversity of Sparganum isolates from snakes in Hunan Province based on mitochondrial nad4 and nad5 genes

Liu, W.

中文

中国血吸虫病防治杂志

1005-6661

2021

33

1

35-39

10.16250/j.32.1374.2020305

湖南省财政厅农业专项养殖业项目（2130199）；

本校为第一机构

目的了解湖南省蛇源裂头蚴种群遗传多样性及其系统发生关系。方法采用PCR技术扩增湖南省7株蛇源裂头蚴线粒体nad4基因和nad5基因部分序列(pnad4和pnad5),扩增产物测序后用DNAMAN 7.0、MegAlign、DnaSP 5.0和MEGA 5.0软件进行同源性和系统进化分析。结果湖南省7株蛇源裂头蚴pnad4和pnad5基因序列长度分别约为578 bp和484 bp,其碱基变异率分别为0~2.8%和0~0.8%;均检测到4个单倍型,总单倍型多样性分别为0.810 ± 0.016和0.905 ± 0.011,核苷酸多样性分别为0.006 ± 0.005和0.004 ± 0.003,平均核苷酸差异为3.960和1.905。构建的系统进化树显示,7株裂头蚴分离株与来自不同地区或不同宿主的猬迭宫绦虫...

Objective Hunan Province. Methods To investigate the genetic diversity and phylogenetic relationship of Sparganum isolates from snakes in The partial mitochondrial NADH dehydrogenase subunit 4 (pnad4) and NADH dehydrogenase sub⁃ unit 5 (pnad5) genes were amplified using a PCR assay in 7 Sparganum isolates from snakes in Hunan Province and the amplifi⁃ cation product was sequenced. The homology and genetic evolution were investigated using the software DNAMAN 7.0, MegA⁃ lign, DnaSP 5.0 and MEGA 5.0. Results The pnad4 and pnad5 gene sequences ...