[Background]Grass carp reovirus(GCRV)infection can cause leakage bleeding by increasing vascu-lar endothelial permeability.[Objective]To investigate the functional mechanism of adenylate cyclase 3(AC3)mediated GCRV infection,a series of studies were conducted in Ctenopharyngodon idella kidney(CIK)cells.[Methods]AC3 overexpression was successfully achieved in CIK cells.Real-time fluorescent quantitative polymerase chain reaction(qPCR)was used to detect the relative expression of ac3,claudin c,claudin 15,claudin 18,irf3,irf7,ifn1 and ifn2 at different time points(0 h,12 h,and 24 h)of GCRV infect...
