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Mutation at Different Sites of Metal Transporter Gene OsNramp5 Affects Cd Accumulation and Related Agronomic Traits in Rice (Oryza sativa L.)

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成果类型:
期刊论文
作者:
Wang, Tiankang;Li, Yixing;Fu, Yuefeng;Xie, Hongjun;Song, Shufeng;...
通讯作者:
Wang, Jianlong;Li, Li
作者机构:
[Wang, Jianlong; Wang, Tiankang] Hunan Agr Univ, Coll Agron, Changsha, Hunan, Peoples R China.
[Qiu, Mudan; Li, Li; Wang, Tiankang; Yuan, Dingyang; Tian, Yan; Li, Chengxia; Li, Yixing; Song, Shufeng] Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha, Hunan, Peoples R China.
[Chen, Muwen; Fu, Yuefeng; Wen, Jiong; Chen, Ge] Yueyang Agr Sci Res Inst, Yueyang, Peoples R China.
[Xie, Hongjun] Hunan Rice Res Inst, Changsha, Hunan, Peoples R China.
通讯机构:
[Wang, Jianlong; Li, Li] H
Hunan Agr Univ, Coll Agron, Changsha, Hunan, Peoples R China.
Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha, Hunan, Peoples R China.
语种:
英文
关键词:
Cd;OsNramp5;quality;rice;yield
期刊:
FRONTIERS IN PLANT SCIENCE
ISSN:
1664-462X
年:
2019
卷:
10
页码:
472897
基金类别:
This work was supported by the Major Scientific Research Project of Hunan Province (Grant No. XCNZ [2018] 83), by the National Natural Science Foundation of China (31671669), by Ministry of Science and Technology of the People’s Republic of China (2017YFD0100400), and by Hunan Provincial Science and Technology Department (2016NK2204). The CRISPR/Cas9 editing vectors, pYLgRNA-U3, and pYLCRISPR/Cas9-MH were provided by Prof. Yaoguang Liu, South China Agricultural University.
机构署名:
本校为第一且通讯机构
院系归属:
农学院
摘要:
OsNramp5 is a key gene involved in the control of the uptake of Cd, Mn, and other metal ions by rice root cells. The functional deficiency of this gene can significantly reduce the accumulation of Cd in rice grains, but the effects of its mutation on agronomic traits such as yield and quality have not been investigated comprehensively yet. In the present study, three Huanghuazhan-based OsNramp5 mutants [LCH1 (Low Cadmium Huanghuazhan 1), LCH2 (Low Cadmium Huanghuazhan 2), and LCH3 (Low Cadmium Huanghuazhan 3)] were obtained using clustered regularly interspaced short palindromic repeats/CRISPR...

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