期刊论文

Effect of Elevated CO_2 and Nitrogen Application on the Abundance of Soil Denitrifying Bacteria in Different Growth Stages of Rice

Xu, H.

中文

环境科学研究

1001-6929

2019

32

4

683-691

10.13198/j.issn.1001-6929.2019.01.05

No.41771300:国家自然科学基金 41771334:国家自然科学基金 No.CARS-01-26:国家水稻产业技术体系项目

本校为第一且通讯机构

随着全球气候变化的不断加剧,大气CO2浓度呈明显增加趋势,这将间接影响土壤-植物-微生物系统的氮循环过程.为研究典型水稻土壤反硝化细菌对CO2浓度升高的响应规律和机制,借助水稻密闭培养箱,运用实时荧光定量聚合酶链式反应（Real-Time q PCR）分子技术,设置不施氮（0 mg/kg）和常规施氮（100 mg/kg） 2个处理,研究CO2倍增对水稻不同生长期土壤关键反硝化功能细菌（nar G、nir K和nir S型）丰度的影响.结果表明:①在2种施氮水平,CO2倍增显著促进了水稻分蘖期、孕穗期、扬花期和成熟期水稻根系生长（增幅为2. 96%～28. 4%）、地上部生物量增加（增幅为7. 1%～107. 3%）以及成熟期籽粒干质量的增加...

The atmospheric CO2 concentration has an obvious rising trend with the increasing global climate change, which will indirectly affect the nitrogen cycling process of the soil-plant-microbe system. In order to investigate the mechanisms of the rising CO2 effect on denitrifying bacteria in paddy soil, we used the airtight rice incubator and the real-time PCR technology to investigate the effect of the elevating CO2 concentration on the abundance of narG-, nirK- and nirS-containing bacterial communities at two nitrogen levels. The results showed t...