The effects of different fertilization systems on the microbes were studied with an 18 year long-term fix position experiment. The soil samples were collected from three sites in Hunan province. The results showed that the organic matter content was not directly correlated to the numbers of bacteria and actinomycetes and could not be used as the indicator for the soil microbes. The quantities of fungi could be used as the indicator for soil fertility based on this study. The nitrogen concentration and nitrifying bacteria in the soils treated with 30% organic matter or traditional fertilization...