In this paper, pure bacterium species was first separated, sublimated and screened out from the muster containingLA, LB and ST. Then rich Selenium(Se) test was processed Separately. Rich Selenium(Se) belonging bacterium species and richSe conditions have been obtained separately by resistant screening and triangle flask fermentation. As a result the tests indicated:Rich Se capability of LA was the best and its rich Se conditions were confirmed: using liquid substrate of tomato, Na2SeO318μg/ml, pH6.6, 37℃, and cultivted 24h. Finally,...