In this work the black kerneled rice was used as raw maternal to extract melanin. The influence of the extaction agent kind, extraction agent concentration, temperature, pH and extraction time on the melanin extraction efficiency were investigated. The results showed that the optimum conditions in extraction were that the melanin was extracted with 70% alcohol （pH2） at 70℃ for 90min each step. The raw product was refined in re-settlement. The yield rate of the refined product was1.4%. The ultraviolet spectrum of the melanin indicetes that the wave length of the maximum absorption is 220hm. It is proposed that the melanin in black kerneled rice might be consisted of enmelanin and pheomelanin with reference to the infrared spectra.