期刊论文

中文

食品科学

1002-6630

2009

30

6

191-195

10.3321/j.issn:1002-6630.2009.06.043

科技部国际科技合作项目(2006DFA33220) “十五”国家科技攻关项目(2004BA542C)

本校为第一机构

优化高速逆流色谱分离4种茶黄素的方法.两相溶剂系统为正己烷-乙酸乙酯-甲醇-水-冰醋酸(1:5:1:5:0.25,V/V),固定相为体系的上相,下相为流动相,流速为2ml/min,仪器转速700r/min,进样量30mg.从茶黄素复合物中分离纯化得到茶黄素、茶黄素-3-没食子酸酯、茶黄素-3'-没食子酸酯和茶黄素-3,3'-双没食子酸酯,尤其使得两种酯溶性茶黄素(茶黄素-3-没食子酸酯、茶黄素-3'-没食子酸酯)达到较好的分离.

In this study, high-speed countercurrent chromatography (HSCCC) was used to separate theaflavins. The optimized two-phase solvent system, flow rate, revolution speed and injection amount of HSCCC were hexane-ethyl acetate- methanol- water-acetic acid (1:5:1:5:0.25, V/V), 2 ml/min, 700 r/min and 30 mg, respectively. The upper phase was used as the stationary phase, while the lower phase was the mobile phase of HSCCC. Under these conditions, four theafl...