期刊论文

中文

食品科学

1002-6630

2011

32

19

165-169

国家“863”计划项目（2007AA10Z347） 湖南省研究生科研创新项目（54040108005/12254）

本校为第一机构

通过平板划线分离法从新鲜牛奶中筛选获得一株乳酸菌菌株,命名为M8,SDS-PAGE分析该菌株富含S-层蛋白。结合表型特征、生理生化特征与16S rRNA基因序列分析法,鉴定该菌株为短乳杆菌（Lactobacillus brevis）。对其人工消化液耐受性分析,结果显示：其对人工胃液（3.0g/L,pH2.5）具有良好耐受能力,能在人工肠液（10g/L,pH8.0）中增殖,且S-层蛋白具有耐消化酶酶解能力。提示S-层蛋白作为保护屏障,可抵抗消化酶的作用。

A strain of lactic acid bacteria was isolated from fresh milk by plate streaking method.The isolated strain was named as M8,which was rich in S-layer protein according to sodium dodecyl sulfonate polyacrylamide gel electrophoresis（SDS-PAGE） analysis.The strain was identified as the species of Lactobacillus brevis via morphological observation,physiological and biochemical characteristics,and phylogenetic analysis of 16S rRNA sequences.Its excellent tolerance was found during sequential suspension at 37 ℃ in a mimic gastric juice（pH 2.5） co...