期刊论文

中文

食品科学

1002-6630

2011

32

18

214-217

(07FJ1005):湖南省重大科技专项

本校为第一机构

建立高效液相色谱同时测定茶叶中8种儿茶素（EGC、DL-C、EC、EGCG、GCG、EGCG4″Me、EGCG3″Me、ECG）、3种生物碱（茶碱、可可碱、咖啡碱）和没食子酸的方法。色谱条件为Welchrom C18（250mm×4.6mm,5μm）色谱柱、磷酸缓冲液-乙腈溶液为流动相、梯度洗脱、波长278nm、流速1.0mL/min、柱温30℃。采用此方法,茶叶中各种成分都能达到基线分离的效果,线性关系良好,具有良好的稳定性和准确度。

A method was developed to determine catechins,purine alkaloids and gallic acid in tea by HPLC-PDA.The optimum condition was achieved by separation on a Welchrom C18 column kept at 30 ℃ using phosphate buffer-acetonitrile as the mobile phase at a flow rate of 1.0 mL/min through gradient elution and detection at 278 nm.This method showed a good linearity for all 12 tested compounds.The results demonst...