期刊论文

中文

食品科学

1002-6630

2012

33

19

310-314

本校为第一机构

目的:探讨灵芝多糖(GLP)联合5-氟尿嘧啶(5-FU)对人结肠癌HCT-116细胞增殖抑制及凋亡的影响。方法:采用MTT法测定单独和联用GLP与5-FU对人结肠癌HCT-116细胞的协同抑制作用,应用联合指数法分析两药物之间相互作用,Hoechst33258荧光染色观察细胞的形态学改变,碘化吡啶(PI)标记流式细胞术(FCM)检测GLP联合5-FU对HCT-116细胞周期和凋亡抑制率的变化。结果:MTT法测定结果显示GLP和5-FU单独用药均能显著抑制HCT-116细胞增殖。GLP(>659μg/mL)联用5-FU(>1.6μg/mL)联用呈协同效应(CI

Objective： To explore the effect of Ganoderma lucidum polysacchrides （GLP） combined with fluorouracil treatment on the proliferation and apoptosis of human colon carcinoma HCT-116 cells in vitro. Methods： The inhibitory effects of both drugs on the proliferation of HCT- 116 cells were measured by using MTT assay. The combinatorial index method was used to analyze their synergistic inhibitory effect. Observations of morphological changes were carried out with Hoechst33258 fluprescemce staining. The impact of GLP on cell cycle and apoptosis r...