期刊论文

Tan, Xinghe(xinghetan@163.com)

中文

食品科学

1002-6630

2016

37

12

13-18

10.7506/spkx1002-6630-201612003

国家自然科学基金面上项目（31371817） 湖南省自然科学基金项目（2015JJ3076）

本校为第一机构

为纯化茅岩莓总黄酮,先用HPD-100型大孔吸附树脂(macroporous adsorption resin,MAR)层析柱进行初步纯化,再用聚酰胺(polyamide,PA)层析柱进行第2次纯化,得到的HPD-100型MAR最适宜吸附工艺参数为上样液总黄酮质量浓度6 mg/mL、上样流速1 mL/min、上样液体积130 mL,在此条件下吸附率为97.14%;最适宜解吸工艺参数为洗脱液乙醇体积分数70%、洗脱流速1 mL/min、洗脱液体积40 mL,在此条件下解吸率为94.10%。经HPD-100型MAR纯化后的总黄酮纯度从55.00%提高到了72.25%。PA的最适宜吸附工艺参数为上样液总黄酮质量浓度6 mg/mL、上样流速2 mL/min,在此条件下吸附率为99.57%;最适宜解吸工艺参数为洗脱液乙...

Sequential column chromatographies on HPD-100 macroporous adsorption resin (MAR) and polyamide (PA) were used to purify total flavonoids from Ampelopsis grossedentata. The optimum adsorption parameters for HPD-100 MAR were determined as follows: sample concentration, 6 mg/mL;sample flow rate, 1 mL/min;and sample volume, 130 mL. Under these conditions, the percentage adsorption was 97.14%. The optimum desorption parameters were established as follows: eluent concentration, aqueous ethanol (70:30, V/V);eluent volume, 40 mL;and flow rate, 1 mL/min. Under these conditions, the percentage desorptio...