期刊论文

Applying Response Surface Methodology to Optimize the Extraction of Neferine from Lotus Seed Core Powder Using Cellulase

Xia, Yanbin(xy520523@aliyun.com)

中文

食品科学

1002-6630

2016

37

10

105-110

10.7506/spkx1002-6630-201610018

湘莲加工技术集成产业化示范项目（2013GA770001）；

本校为第一机构

以莲子钻芯粉为原料,采用纤维素酶辅助提取莲子钻芯粉中的甲基莲心碱,在单因素试验的基础上,选择加酶量、酶解温度、酶解时间、酶解pH值为自变量,以甲基莲心碱提取量为响应值,通过Box-Behnken响应面设计优选最佳工艺条件。结果表明:采用纤维素酶辅助提取莲子钻芯粉中甲基莲心碱,最佳提取工艺条件为加酶量3.1%(质量分数)、酶解时间1.7 h、酶解pH 4.8、酶解温度50 ℃。该工艺条件下得到的甲基莲心碱提取量为(7.02±0.22)mg/g,高于传统乙醇法(4.03±0.17)mg/g,提取量提高了71.9%。采用纤维素酶辅助提取法操作简单,条件温和,有效地提高了莲子钻芯粉的利用率。

The cellulase-assisted extraction of neferine from lotus seed core powder was optimized using single-factor experiments and response surface methodology based on Box-Behnken design. The independent variables were cellulase dosage, hydrolysis pH, temperature and time. Neferine yield was used as the response. The extraction conditions were optimized as follows: cellulase dosage, 3.1% (m/m);pH, 4.8;hydrolysis time, 100 min;and hydrolysis temperature, 50 ℃. Under the optimized conditions, the yield of neferine was (7.02 ±0.22) mg/g, which was 71.9% higher than that ((4.03 ±0.17...