期刊论文

Wang, Yan(693675748@qq.com)

中文

食品科学

1002-6630

2017

38

18

244-250

10.7506/spkx1002-6630-201718038

湖南省重金属污染耕地治理修复机理及技术模式优化集成项目

本校为第一机构

以微波预糊化籼米淀粉为原料,自制RS_3型马铃薯抗性淀粉为晶种,研究RS_3型籼米抗性淀粉的晶种诱导-双酶复合法制备工艺。利用扫描电子显微镜对淀粉颗粒形貌进行表征并研究淀粉的抗酶解性。在单因素试验的基础上,固定其他酶解条件,以RS_3型籼米抗性淀粉产率为响应值,确定晶种添加量、异淀粉酶添加量、普鲁兰酶添加量和普鲁兰酶酶解时间作为影响产率的主要因素,进行Box-Behnken响应面优化试验。得到RS_3型籼米抗性淀粉的最佳制备工艺条件为:晶种添加量5%、异淀粉酶添加量8 U/g、普鲁兰酶添加量8 U/g、普鲁兰酶酶解时间3.50 h。在此最佳制备工艺条件下,RS_3型籼米抗性淀粉产率为27.42%,RS_3失去原有...

In this research, RS3type resistant potato starch was prepared and used as seed crystal to investigate the preparation of RS3type resistant indica rice starch by the combined use of seed crystal and sequential hydrolysis with isoamylase and pullulanase. Scanning electron microscopy (SEM) was used to characterize starch granule morphology. Besides, we also studied the resistance of starches to enzymatic hydrolysis. Using the one-factor-at-a-time method, the amounts of seed crystal, isoamylase and pullulanase added and pullulanase hydrolysis time were identified as factors with a significant inf...