期刊论文

中文

食品科学

1002-6630

2018

39

13

229-234

10.7506/spkx1002-6630-201813034

CX2017B357:湖南省研究生科研创新项目 :猕猴桃镉低积累品种筛选 5026301/111502600009:配套技术研究与示范项目 16B188:湖南省教育厅优秀青年项目

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研究软枣猕猴桃中黄酮类化合物对过氧化氢(H_2O_2)损伤人永生化表皮细胞(HaCaT细胞)的保护作用。以湖南省浏阳市大围山软枣猕猴桃为实验材料,以体外培养的HaCaT细胞为实验对象。实验分为空白对照组、H_2O_2模型组、阳性对照组(VC组)和黄酮处理组(软枣猕猴桃黄酮0.1~1.0 mg/mL预处理),以细胞内超氧化物歧化酶(superoxide dismutase,SOD)活力、活性氧簇(reactive oxygen species,ROS)水平为综合评价指标,并以VC作为阳性对照评价其抗氧化活性。实验结果表明:当H_2O_2浓度为30 μmol/L时,细胞相对存活率为40%左右,较好地诱导了HaCaT细胞氧化损伤模型。当加入黄酮类化合物对细胞进行预保护后,质量浓度...

The protective effect of Actinidia arguta flavonoids (AAF) on H_2O_2-induced oxidative injury in human immortalized epidermal cells (HaCaT) was investigated in this study. The flavonoids were extracted from A. arguta grown in DaWei Mountain, Liuyang, Hunan provice. HaCaT cells were cultured in vitro. The experiment was divided into control, hydrogen peroxide damage, positive control (VC), and AAF treatment groups (at concentrations of 0.1–1.0 mg/mL). Superoxide dismutase (SOD) activity and reactive oxygen species (ROS) levels in cells were mea...