期刊论文

Protective Effect of Betulinic Acid on Intestinal Oxidative Damage Induced by T-2 Toxin in Mice

中文

食品科学

1002-6630

2020

41

11

194-200

10.7506/spkx1002-6630-20190603-019

17A098:湖南省教育厅重点项目 CX2018B411:湖南省研究生科研创新项目 2017JJ3108:湖南省自然科学基金

本校为第一机构

采用腹腔注射T-2毒素诱导小鼠肠道氧化损伤模型,研究桦木酸(betulinic acid,BA)对肠道氧化损伤的保护作用。60只健康雄性KM小鼠随机6组,分别为对照组,T-2毒素组,BA低、中、高剂量(0.25、0.5、 1 mg/kg mb)+T-2毒素组,VE+T-2毒素组。BA连续灌胃14 d后,检测血清中免疫球蛋白(immunoglobulin, Ig)G、IgM和二胺氧化酶(diamine oxidase,DAO)活性,十二指肠、空肠和回肠等小肠组织中过氧化氢酶(catalase,CAT)和谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活性,以及谷胱甘肽(glutathione, GSH)和丙二醛(malondialdehyde,MDA)含量的变化,苏木精-伊红染色观察空肠形态结构的改变,探究BA对肠道氧化...

In order to explore the protective effect of betulinic acid (BA) on T-2 toxin-induced intestinal oxidative damage, 60 healthy male mice were randomly divided into 6 equal groups: control, T-2 toxin, low-, medium- and high-dose BA (0.25, 0.5 and 1 mg/kg mb) plus T-2, and vitamin E plus T-2. After BA was administered for 14 days, the levels of immunoglobulin (Ig)M, IgG, and diamine oxidase (DAO) in serum were detected by enzyme-linked immunosorbent assay, the activities of catalase (CAT) and glutathione peroxidase (GSH-Px), and the contents of malondialdehyde (MDA) and glutathione (GSH) in the d...