期刊论文

Diversity and Seasonal Variation of Bacteria Contaminating Fresh Duck Egg Surface

中文

食品科学

1002-6630

2021

42

18

306-313

10.7506/spkx1002-6630-20201201-016

16A099:湖南省教育厅重点科研项目 201303084:公益性行业(农业)科研专项 20C0959:湖南省教育厅一般项目

本校为第一机构

为研究鸭蛋壳表面污染细菌的多样性以及季节性变化,在春、夏、秋和冬4个季节采集新鲜鸭蛋,利用超声波洗涤蛋壳制备细菌样本,通过Illumina MiSeq高通量测序分析样品细菌菌群的多样性。结果表明,共获得697 244条优化序列归属于650个操作分类单元(operational taxonomic units,OTU),包括35个门,298个属,447个种。其中相对丰度占比较大的优势菌有:厚壁菌门(Firmicutes)芽孢杆菌属(Bacillus)和肠球菌属(Enterococcus),分别占比17.78%和17.16%;变形菌门(Proteobacteria)肠杆菌科(Enterobacteriaceae)埃希氏菌属-志贺菌属(Escherichia-Shigella)占比17.14%。4个季节样品的优势菌群相同,皆为芽孢杆菌属(...

To study the diversity and seasonal variation of bacteria contaminating duck eggshell surfaces, fresh duck eggs collected in different seasons were cleaned by ultrasonic washing to prepare bacterial samples. The diversity of bacterial flora in the samples was analyzed by Illumina MiSeq high-throughput sequencing. The results showed that a total of 697 244 optimal sequences were obtained and assigned to 650 operational taxonomic units (OTU), covering 35 phyla, 298 genera, and 447 species. For each seasonal samples, the dominant bacteria with a h...