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Morphological and molecular characterization of two G. somalense monosomic alien addition lines (MAALs)

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成果类型:
期刊论文
作者:
Zhou, ZH;Yu, P;Liu, GH;He, JX*;Chen, JX;...
通讯作者:
He, JX
作者机构:
[He, JX] Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China.
Hunan Agr Univ, Inst Cotton, Changsha 410128, Peoples R China.
通讯机构:
[He, JX] C
Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China.
语种:
英文
关键词:
RAPD;MAALs;G.somalense;G.hirsutum;G. hirsutum;G. somalense;MALLs;morphology;cytology;RAPD.
关键词(中文):
细胞学;形态学;单染色体异类附加线;棉属
期刊:
科学通报(英文版)
ISSN:
2095-9273
年:
2004
卷:
49
期:
9
页码:
910-914
基金类别:
Acknowledgements The authors would like to thank Dr. D. Wang for help in writing. This work was supported by the National Natural Science Foundation of China (Grant Nos. 30070482 and 30170560), the "863" Program (Grant No. 200IAA2410813) and the project ofthe Chinese Academy of Sciences (Grant No. KSCX2-1-01-2-06).
机构署名:
本校为其他机构
院系归属:
农学院
摘要:
Two G. somalense monosomic alien addition lines were identified from the derived backcross progenies of allohexaploid between G. hirsutum and G. somalense through cytological and morphological observation. Furthermore, the alien addition chromosome was identified and distinguishedby RAPD analysis. A total of 160 RAPD primers were usedfor PCR amplification. Primer SBSG11 could produce a specific molecular marker (600 bp) for monosomic alien addition line Ⅰ (MAAL Ⅰ ). Primer SBSC03 could produce aspecific molecular marker (700 bp) for monosomic...

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