期刊论文

Xiao LangTao

英文

科学通报(英文版)

2095-9273

2010

55

21

2231-2235

10.1007/s11434-010-3194-4

The authors would like to thank Prof. Xie Daoxin at Tsinghua University who donated some chemicals. This work was supported by the National Natural Science Foundation of China (90817101, 30670190 and 30600049).

本校为第一且通讯机构

An efficient method for the simultaneous extraction and quantification of both jasmonic acid (JA) and methyl jasmonate (MeJA) from a single plant sample was developed. Plant tissues were firstly extracted by using 100% cold methanol, then the Sep-pak C18 solid-phase extraction (SPE) cartridges were adopted for the purification of sample extract, and finally JA and MeJA were determined by the liquid chromatography tandem mass spectrometry (LC-MS-MS) system. It was found that the accuracy was improved by using the extra standard for the estimatio...

为 jasmonic 酸(JA ) 和从一件单个工厂样品的甲基 jasmonate (MeJA ) 的同时的抽取和 quantification 的一个有效方法被开发。植物纸巾被使用 100% 寒冷甲醇第一提取，然后， Sep-pak C18 稳固阶段的抽取(SPE ) 子弹为样品摘录的纯化被采用，并且最后， JA 和 MeJA 被液体层析双人脚踏车团 spectrometry (LC-MS-MS ) 决定系统。精确性被在抽取和纯化过程为 JA 和 MeJA 损失的评价和修正使用额外的标准改进，这被发现。为 JA 和 MeJA 的察觉限制是 0.03 和 0.075 ng mL ? 1，分别地并且 JA 和 MeJA 的平均恢复率是 92.48% 和 94.30% 。方法被发现可再现、选择，产出孤立的井和为 JA 和 MeJA 的容...