Characteristics of Metabolites by Seed-Specific Inhibition of FAD2 in Brassica napus L

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成果类型：

期刊论文

作者：

Zhou, Chi;Pan, Weisong;Peng, Qi;Chen, Yanchao;Zhou, Ting;...

通讯作者：

Liqun Rao<&wdkj&>Qiming Wang

作者机构：

[Chen, Yanchao; Zhou, Chi; Pan, Weisong; Wang, Qiming; Zhou, Ting; Xu, Minhui; Liu, Chuwei; Rao, Liqun] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China.

[Chen, Yanchao; Zhou, Chi; Wang, Qiming; Zhou, Ting; Xu, Minhui; Liu, Chuwei; Rao, Liqun] Hunan Agr Univ, Hunan Engn Lab Good Agr Practice & Comprehens Uti, Changsha 410128, Peoples R China.

[Peng, Qi] Jiangsu Acad Agr Sci, Inst Ind Crops, Key Lab Cotton & Rapeseed, Minist Agr, Nanjing 210014, Peoples R China.

[Peng, Qi] Jiangsu Univ, Inst Life Sci, Zhenjiang 212013, Peoples R China.

[Wu, Chuan] Cent South Univ, Sch Met & Environm, Changsha 410083, Peoples R China.

通讯机构：

[Liqun Rao; Qiming Wang] C

College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Hunan Engineering Laboratory for Good Agricultural Practice and Comprehensive Utilization of Famous-Region Medicinal Plants, Hunan Agricultural University, Changsha 410128, China

语种：

英文

关键词：

Brassica napus L.;Rnai;metabolomics;fatty acid desaturase-2

期刊：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

ISSN：

0021-8561

年：

2021

卷：

期：

页码：

5452-5462

DOI：

10.1021/acs.jafc.0c06867

基金类别：

National Science and Technology Major Project of China [2018ZX08011-01B]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31771834]; Natural Science Foundation of Jiangsu ProvinceNatural Science Foundation of Jiangsu Province [BK20191237]

机构署名：

本校为第一机构

院系归属：

农学院

生物科学技术学院

摘要：

Fatty acid desaturase-2 (FAD2) is a key enzyme in the production of polyunsaturated fatty acids in plants. RNAi technology can reduce the expression of FAD2 genes in Brassica napus seeds and acquire transgenic B. napus plants with a high oleic acid content, but the effect of seed-specific inhibition of FAD2 expression on B. napus seed metabolites is not clear. Here we use widely targeted metabolomics to investigate the metabolites of normal-oleic-acid rapeseed (OA) and high-oleic-acid rapeseed (HOA) seeds, resulting in a total of 726 metabolites being detected. Among them, 24 differential meta...

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Characteristics of Metabolites by Seed-Specific Inhibition of FAD2 in Brassica napus L

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