期刊论文

中文

农业环境科学学报

1672-2043

2010

29

2

396-399

浙江省农科院创新能力提升项目 国家自然科学基金（50808072） ***和创新团队发展计划资助（IRT0719） 浙江省自然科学基金（Y507284）

本校为第一机构

用限制性片段长度多态性(Restriction fragment length polymorphism,RFLP)方法研究了在农业废物堆肥一次发酵和二次发酵期间添加黄孢原毛平革菌(P.chrysosporium)对微生物多样性的影响.结果表明,在不同发酵期接种P.chrysosporium对堆肥进程的影响不同.3种典型的限制性内切酶Alu Ⅰ、Hae Ⅲ和Taq Ⅰ在分析堆肥细菌微生物多样性的灵敏性上,Hae Ⅲ效果最好,Alu Ⅰ次之,Taq Ⅰ则并不很适用于分析堆肥细菌微生物多样性.接种黄孢对堆肥细菌群落的影响非常显著且迅速,二次接种可起到巩固一次接种效果的作用.

The lignocellulolytic microorganism, P. chrysosporium was inoculated during different phases of agricultural waste composting and the effect on the diversity of microbial community was observed. The results of the present investigation revealed that inoculation during different phases had different effects on these parameters. Three kinds of restriction enzymes, Alu Ⅰ, Hae Ⅲ and Taq Ⅰ were used to analyse the change of microbial community diversity. The results showed that restriction enzyme Hae Ⅲ was the most sensitive to the change of mic...